2XLA
Structure and metal-loading of a soluble periplasm cupro-protein: Cu- CucA-closed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.310, 72.510, 262.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.420 - 1.930 |
| R-factor | 0.16347 |
| Rwork | 0.161 |
| R-free | 0.20318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xl9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.432 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.030 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.092 | 0.250 |
| Number of reflections | 70481 | |
| <I/σ(I)> | 9.4 | 4.1 |
| Completeness [%] | 96.0 | 92 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M TRIS PH8.0, 20% PEG 8000 |
