2XL7
Structure and metal-loading of a soluble periplasm cupro-protein: Cu- CucA-closed (SeMet)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 88.483, 88.483, 192.419 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.240 - 2.400 |
| R-factor | 0.17609 |
| Rwork | 0.174 |
| R-free | 0.21317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xl9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.340 |
| Number of reflections | 18072 | |
| <I/σ(I)> | 13.6 | 4.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 20% (W/V) PEG 8000, 0.5MM CUSO4 |
