2XL6
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124A variant with bound NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.940, 52.940, 182.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.830 - 1.070 |
| R-factor | 0.13746 |
| Rwork | 0.136 |
| R-free | 0.16062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xlh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.749 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.100 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 67775 | |
| <I/σ(I)> | 22 | 3.8 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | AMMONIUM SULPHATE, TRIS PH7.5 |
