2XL6
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124A variant with bound NO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 52.940, 52.940, 182.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.830 - 1.070 |
R-factor | 0.13746 |
Rwork | 0.136 |
R-free | 0.16062 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xlh |
RMSD bond length | 0.017 |
RMSD bond angle | 1.749 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.100 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmerge | 0.050 | 0.480 |
Number of reflections | 67775 | |
<I/σ(I)> | 22 | 3.8 |
Completeness [%] | 99.1 | 99.3 |
Redundancy | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | AMMONIUM SULPHATE, TRIS PH7.5 |