2XL4
LntA, a virulence factor from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 49.672, 49.672, 141.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.850 - 2.300 |
| R-factor | 0.23323 |
| Rwork | 0.232 |
| R-free | 0.25521 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.972 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.830 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 8491 | |
| <I/σ(I)> | 24.9 | 7 |
| Completeness [%] | 96.4 | 90.3 |
| Redundancy | 13.6 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M NASO4 18% PEG 3350 |
