2XKQ

Crystal structure of Streptococcus suis Dpr with manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	106.222, 139.467, 143.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.400
R-factor	0.18611
Rwork	0.183
R-free	0.24490
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.333
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.4.0078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.490
Number of reflections	83975
<I/σ(I)>	14.1	2.8
Completeness [%]	99.9	99.9
Redundancy	5.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		30 - 35 % (V/V) PEG 400, 0.2 M CACL2, 0.1 M HEPES-NAOH (PH 7.4)