2XKB
Crystal structure of GDP-form protofilaments of Bacillus thuringiensis serovar israelensis TubZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.947, 541.099, 86.128 |
| Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.23729 |
| Rwork | 0.234 |
| R-free | 0.29431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xka |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.660 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 89306 | |
| <I/σ(I)> | 10.2 | 2.5 |
| Completeness [%] | 92.9 | 65.4 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRISCL PH 8.5 200 MM MGCL2 30% PEG4000 |
