2XJW
Lysozyme-CO releasing molecule adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-07-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.400, 78.400, 36.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.760 - 1.670 |
| R-factor | 0.17035 |
| Rwork | 0.169 |
| R-free | 0.20763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.760 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 13925 | |
| <I/σ(I)> | 24.1 | 6.2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 10.7 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 2-10% (M/V) NACL, 0.1 M ACETATE BUFFER PH 4.5. HANGING AND SITTING DROPS |
