2XJV
X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae with mutation D201T in complex with calcium and glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-23 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.450, 62.360, 106.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.360 - 1.740 |
| R-factor | 0.18191 |
| Rwork | 0.181 |
| R-free | 0.21855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED STRUCTURAL DATA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.420 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.120 | 0.660 |
| Number of reflections | 32352 | |
| <I/σ(I)> | 12.6 | 2.8 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5 M NACL, 0.1M BISTRIS-PROPANE PH7.5, 20% PEG4000 |
