2XJP
X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.280, 61.820, 106.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.950 |
| R-factor | 0.10553 |
| Rwork | 0.105 |
| R-free | 0.12236 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.071 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX (CDE) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.290 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.030 | 0.440 |
| Number of reflections | 190329 | |
| <I/σ(I)> | 19.6 | 2.8 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5 M NACL, 0.1 M BISTRIS PH7.5, 20% PEG 4000 |
