2XJN

Crystal structure of Streptococcus suis Dpr with copper

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-10
Detector	MARRESEARCH MARMOSAIC
Spacegroup name	P 21 21 21
Unit cell lengths	104.840, 137.710, 142.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.760 - 2.100
R-factor	0.16225
Rwork	0.160
R-free	0.21101
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FRIEDEL_LAW=FALSE WAS USED DURING DATA PROCESSING AND A TOTAL OF 1225754 REFLECTIONS WERE PROCESSED.
RMSD bond length	0.014
RMSD bond angle	1.345
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.4.0078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.350
Number of reflections	118636
<I/σ(I)>	11.8	2.7
Completeness [%]	97.3	79
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		30 - 35 % (V/V) PEG 400, 0.2 M CACL2, 0.1 M HEPES-NAOH (PH 7.4)