2XJ9

Dimer Structure of the bacterial cell division regulator MipZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-16
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41
Unit cell lengths	57.122, 57.122, 164.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.847 - 2.800
R-factor	0.1881
Rwork	0.185
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xj4
RMSD bond length	0.010
RMSD bond angle	1.530
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.350
Number of reflections	10750
<I/σ(I)>	8.9	2.7
Completeness [%]	94.2	96.5
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		20 MM TRIS PH 8.5, 23.6% ETHANOL