2XJ4
Structure of the bacterial cell division regulator protein MipZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.334, 81.334, 124.206 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.310 - 1.600 |
| R-factor | 0.12863 |
| Rwork | 0.126 |
| R-free | 0.17531 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.474 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.690 | 1.690 |
| High resolution limit [Å] | 1.500 | 1.600 |
| Rmerge | 0.090 | 0.290 |
| Number of reflections | 40200 | |
| <I/σ(I)> | 11.6 | 5.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES/NAOH PH6.5, 30% V/V PEG400 |
