2XIS
A METAL-MEDIATED HYDRIDE SHIFT MECHANISM FOR XYLOSE ISOMERASE BASED ON THE 1.6 ANGSTROMS STREPTOMYCES RUBIGINOSUS STRUCTURES WITH XYLITOL AND D-XYLOSE
Experimental procedure
Spacegroup name | I 2 2 2 |
Unit cell lengths | 94.640, 99.970, 103.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.710 |
R-factor | 0.149 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.031 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.710 * |
Total number of observations | 291153 * |
Number of reflections | 45797 * |
Completeness [%] | 90.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 8.0 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 21 (%sat) | |
3 | 1 | drop | 0.5 (mM) | ||
4 | 1 | drop | HEPES | 50 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 43 (%sat) | |
6 | 1 | reservoir | HEPES | 50 (mM) |