2XIO
Structure of putative deoxyribonuclease TATDN1 isoform a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.480, 50.940, 155.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.420 - 1.190 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.550 | 1.250 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.020 | 0.560 |
| Number of reflections | 94152 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 95.4 | 79.8 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MES 6.5;12% W/V PEG 20K |
