2XI1

Crystal structure of the HIV-1 Nef sequenced from a patient's sample

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 63 2 2
Unit cell lengths	123.030, 123.030, 130.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.551 - 3.500
R-factor	0.2268
Rwork	0.224
R-free	0.28800
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.393
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.900
High resolution limit [Å]	3.500
Rmerge	0.120
Number of reflections	7262
<I/σ(I)>	7.1
Completeness [%]	93.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1