2XHN
Rhamnogalacturonan lyase from Aspergillus aculeatus K150A active site mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.934, 108.978, 170.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.007 - 1.520 |
| R-factor | 0.1159 |
| Rwork | 0.113 |
| R-free | 0.16440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AUTOMR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.060 | 0.150 |
| Number of reflections | 142062 | |
| <I/σ(I)> | 24 | 9.7 |
| Completeness [%] | 91.6 | 86 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 20% PEG 4000, 0.1 AMMONIUM SULFATE, HANGING DROP VAPOR DIFFUSION |
