2XH8
X-ray structure of 119-141 ZnuA deletion mutant from Salmonella enterica.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 143.514, 143.514, 30.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.080 |
| R-factor | 0.19687 |
| Rwork | 0.195 |
| R-free | 0.22522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prs |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.130 | 0.430 |
| Number of reflections | 22468 | |
| <I/σ(I)> | 18 | 4 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULFATE 2.3-2.5 M, PEG 400 1-2 %, HEPES 0.1 M PH 7.5 . |
