2XH3
extracellular nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.822, 101.822, 113.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.550 - 2.490 |
| R-factor | 0.1706 |
| Rwork | 0.167 |
| R-free | 0.24414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xgr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.831 |
| Data reduction software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.900 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.480 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 18955 | |
| <I/σ(I)> | 17.5 | 4.5 |
| Completeness [%] | 90.3 | 82.5 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M NA CITRATE, 0.1M BIS-TRIS PROPANE PH 8.5, 20% PEG 3350 |
