2XGY
Complex of Rabbit Endogenous Lentivirus (RELIK)Capsid with Cyclophilin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.158, 117.158, 49.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.482 - 1.800 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.931 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.600 |
| Number of reflections | 35238 | |
| <I/σ(I)> | 71.4 | 4.4 |
| Completeness [%] | 96.8 | 94.1 |
| Redundancy | 21.2 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 16-26% PEG3350, 0.1M HEPES PH 7.5 |
