2XGT
Asparaginyl-tRNA synthetase from Brugia malayi complexed with the sulphamoyl analogue of asparaginyl-adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.510, 125.700, 144.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.920 - 1.900 |
| R-factor | 0.20378 |
| Rwork | 0.202 |
| R-free | 0.25017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T. THERMOPHILUS ASPARAGINYL-TRNA SYNTHETASE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 71529 | |
| <I/σ(I)> | 14.9 | 1.9 |
| Completeness [%] | 89.5 | 69.6 |
| Redundancy | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100MM HEPES PH8.5, 200MM NA-ACETATE, 14-16% PEG 4000 |
