2XGJ

Structure of Mtr4, a DExH helicase involved in nuclear RNA processing and surveillance

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	298
Detector technology	PIXEL
Collection date	2009-11-30
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	100.110, 126.019, 102.272
Unit cell angles	90.00, 99.21, 90.00

Refinement procedure

Resolution	53.453 - 2.900
R-factor	0.203
Rwork	0.201
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IN HOUSE SE-MET MAD STRUCTURE
RMSD bond length	0.002
RMSD bond angle	0.623
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.400	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.110	0.540
Number of reflections	55417
<I/σ(I)>	11.3	3.9
Completeness [%]	99.7	100
Redundancy	5.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		PROTEIN WAS CRYSTALLISED IN 50 MM MES PH 6.0, 200 MM AMMONIUM ACETATE, 20 % (W/V) PEG3350. 15% ETHYLENE GLYCOL WAS USED AS A CRYOPROTECTANT.