2XGG

Structure of Toxoplasma gondii Micronemal Protein 2 A_I Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 43 21 2
Unit cell lengths	63.034, 63.034, 157.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.840 - 2.050
R-factor	0.20991
Rwork	0.206
R-free	0.27827
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.023
RMSD bond angle	2.045
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.840	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.060	0.430
Number of reflections	20754
<I/σ(I)>	15.9	4.7
Completeness [%]	100.0	100
Redundancy	10.69	10.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				27% PEG3350, 0.1M HEPES 7.5, 0.2M AMMONIUM SULFATE