2XFD
vCBM60 in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2009-04-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.610, 41.990, 80.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.040 - 1.190 |
| R-factor | 0.11854 |
| Rwork | 0.117 |
| R-free | 0.14795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO-VCBM60 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.890 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.040 | 1.220 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.060 | 0.180 |
| Number of reflections | 30656 | |
| <I/σ(I)> | 12.9 | 6.7 |
| Completeness [%] | 98.4 | 98.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.4 M SODIUM MALONATE, 10 MM CELLOTRIOSE, pH 8 |
