2XFA

Crystal structure of Plasmodium berghei actin depolymerization factor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	111.580, 57.900, 40.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.100
R-factor	0.1895
Rwork	0.186
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1F7S 1ahq AND 2I2Q
RMSD bond length	0.008
RMSD bond angle	1.098
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.650
Number of reflections	15859
<I/σ(I)>	16.4	4.2
Completeness [%]	99.1	99.8
Redundancy	6.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.2	277	26 % PEG 10000, 0.1 M SODIUM ACETATE PH 5.2, 277 K