2XEX
crystal structure of Staphylococcus aureus elongation factor G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2009-07-24 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.160, 137.340, 125.360 |
| Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
| Resolution | 46.200 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnm |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.555 |
| Number of reflections | 110987 | |
| <I/σ(I)> | 16.5 | 3.1 |
| Completeness [%] | 92.7 | 78.5 |
| Redundancy | 3.71 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
