2XEC
Nocardia farcinica maleate cis-trans isomerase bound to TRIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.160, 84.510, 238.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.670 - 2.200 |
| R-factor | 0.24112 |
| Rwork | 0.239 |
| R-free | 0.28841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dg9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.697 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.160 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 52302 | |
| <I/σ(I)> | 9.6 | 2.6 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 300 MM CA ACETATE 100 MM TRIS PH 7.5 5 MM NA MALEATE 16% PEG 3350 |
