2XE7
The complete reaction cycle of human phosphoglycerate kinase: The open ternary complex with 3PG and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.620, 73.450, 93.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.22757 |
| Rwork | 0.224 |
| R-free | 0.28829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c39 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.621 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 20841 | |
| <I/σ(I)> | 12.8 | 4 |
| Completeness [%] | 99.1 | 99.8 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 30% PEG4000, 100MM TRIS PH 8.0 |
