2XE2
Molecular insights into clinically isolated OmpC20 mutants and their role in multi-drug resistance
Replaces: 2IXWExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.023, 74.198, 133.499 |
| Unit cell angles | 90.00, 124.75, 90.00 |
Refinement procedure
| Resolution | 109.760 - 2.500 |
| R-factor | 0.21873 |
| Rwork | 0.217 |
| R-free | 0.25651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xe1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.991 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0060) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.630 |
| Number of reflections | 33821 | |
| <I/σ(I)> | 13 | 1.7 |
| Completeness [%] | 99.0 | 96.7 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
