2XD7
Crystal structure of the macro domain of human core histone H2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.030, 61.640, 242.230 |
| Unit cell angles | 90.00, 89.64, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.090 |
| R-factor | 0.21922 |
| Rwork | 0.217 |
| R-free | 0.25298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1YD9 1zr3 1zr5 3iid |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.860 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 45536 | |
| <I/σ(I)> | 7.5 | 2.3 |
| Completeness [%] | 92.7 | 77.7 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.05M MGCL2; 0.1M HEPES PH 7.5; 30% MPEG 550 |
