2XCM

COMPLEX OF HSP90 N-TERMINAL, SGT1 CS AND RAR1 CHORD2 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-13
Detector	ADSC CCD
Spacegroup name	P 32
Unit cell lengths	88.890, 88.890, 117.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.780 - 2.200
R-factor	0.2
Rwork	0.198
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jkl
RMSD bond length	0.008
RMSD bond angle	1.219
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.370	3.160
High resolution limit [Å]	2.500	3.000
Rmerge	0.070	0.340
Number of reflections	52431
<I/σ(I)>	8.39	2.22
Completeness [%]	98.5	98.6
Redundancy	2.68	2.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.1M HEPES PH 7.5 23% PEG5000 MME