2XC8
Crystal structure of the gene 22 product of the Bacillus subtilis SPP1 phage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.911, 64.116, 101.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.680 - 2.350 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.24360 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | BUSTER-TNT (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.140 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 12165 | |
| <I/σ(I)> | 27.3 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 7.2, 0.01 M COCL2, 2.05 M AMMONIUM SULFATE |
