2XBL
Crystal structure of GmhA from Burkholderia pseudomallei in complex with product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-21 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9763,1.283 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.200, 83.680, 126.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.772 - 1.620 |
| R-factor | 0.148 |
| Rwork | 0.146 |
| R-free | 0.17900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCDE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 1.710 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 93361 | |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 94.4 | 71.8 |
| Redundancy | 6.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.05 M SODIUM ACETATE PH 4.6, 4% PEG 4000 |
