2XBB
Nedd4 HECT:Ub complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.188, 49.276, 132.627 |
| Unit cell angles | 90.00, 108.88, 90.00 |
Refinement procedure
| Resolution | 43.590 - 2.680 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2XBF AND 1UBI |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_542)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.090 | 0.380 |
| Number of reflections | 29856 | |
| <I/σ(I)> | 21.2 | 3.9 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 6.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM NA-HEPES, PH 7.5, 10% PEG 2000 MME, 5 MM TCEP. |
