2XB5

Tet repressor (class D) in complex with 7-Iodotetracycline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-04-01
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	68.860, 68.860, 180.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.550 - 2.500
R-factor	0.16847
Rwork	0.162
R-free	0.23018
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.612
Data reduction software	MOSFLM
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	64.550
High resolution limit [Å]	2.450
Rmerge	0.060
Number of reflections	7120
Completeness [%]	99.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2		10MICROL PROTEIN (0.2MM TETR, 1MM IODOTC, 5MM MGCL2), 5MICROL RESERVOIR (35% AMMONIUM SULPHATE, TRIS-HCL PH 7.2) VAPOUR DIFFUSSION HANGING DROP