2XB3
The Structure of Cyanobacterial PsbP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.946, 64.946, 81.806 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.080 - 2.800 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.618 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.310 | 2.950 |
| High resolution limit [Å] | 2.500 | 2.800 |
| Rmerge | 0.110 | 0.450 |
| Number of reflections | 5133 | |
| <I/σ(I)> | 5.59 | 1.47 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 6.81 | 7.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M ZN ACETATE, 0.1M NA-CACODYLATE PH 6.5, 10% V/V 2-PROPANOL |
