2XAP
Ribonucleotide reductase Y731NO2Y modified R1 subunit of E. coli to 2. 1 A resolution
Replaces: 2X1YExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-29 |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 223.979, 223.979, 336.027 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 169.031 - 2.100 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x0x |
RMSD bond length | 0.008 |
RMSD bond angle | 1.041 |
Data reduction software | MOSFLM |
Data scaling software | TRUNCATE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.990 | 2.120 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.470 |
Number of reflections | 177593 | |
<I/σ(I)> | 17.15 | 3.34 |
Completeness [%] | 95.2 | 96.8 |
Redundancy | 8.17 | 8.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | LITHIUM SULPHATE 1.5M, SODIUM CHLORIDE BUFFER PH 6. |