2X9X

STRUCTURE OF THE PILUS BACKBONE (RRGB) FROM STREPTOCOCCUS PNEUMONIAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 61 2 2
Unit cell lengths	142.563, 142.563, 89.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.665 - 1.500
R-factor	0.1696
Rwork	0.168
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x9w
RMSD bond length	0.004
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.910
Number of reflections	85582
<I/σ(I)>	35.9	1.3
Completeness [%]	99.7	98.3
Redundancy	9.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN WAS CRYSTALLIZED FROM 0.05 POTASSIUM DIHYDROGEN PHOSPHATE, 20% PEG-8000 PH 4.5