2X9W
STRUCTURE OF THE PILUS BACKBONE (RRGB) FROM STREPTOCOCCUS PNEUMONIAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.360, 74.063, 104.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.977 - 1.920 |
| R-factor | 0.1764 |
| Rwork | 0.174 |
| R-free | 0.22760 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.920 |
| Rmerge | 0.070 | 0.930 |
| Number of reflections | 40524 | |
| <I/σ(I)> | 26 | 0.95 |
| Completeness [%] | 99.4 | 89.3 |
| Redundancy | 13 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS CRYSTALLIZED FROM 0.05 POTASSIUM DIHYDROGEN PHOSPHATE, 20% PEG-8000, PH 4.5 |
