2X9O
STRUCTURE OF 15, 16- DIHYDROBILIVERDIN:FERREDOXIN OXIDOREDUCTASE (PebA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.420, 39.350, 71.270 |
| Unit cell angles | 90.00, 105.64, 90.00 |
Refinement procedure
| Resolution | 40.185 - 1.550 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.22470 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.164 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.180 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 33089 | |
| <I/σ(I)> | 11.53 | 2.91 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100MM HEPES PH 7.0, 25% PEG4000 |
