2X8U

Sphingomonas wittichii Serine palmitoyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 31
Unit cell lengths	99.370, 99.370, 72.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.320 - 2.100
R-factor	0.16437
Rwork	0.163
R-free	0.19612
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.109
Refinement software	REFMAC (5.6.0060)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.480
Number of reflections	44612
<I/σ(I)>	16	3
Completeness [%]	99.0	99
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1