2X86
AGME bound to ADP-B-mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU CCD RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 138.074, 162.458, 185.027 |
| Unit cell angles | 90.00, 101.45, 90.00 |
Refinement procedure
| Resolution | 35.050 - 2.800 |
| R-factor | 0.25321 |
| Rwork | 0.252 |
| R-free | 0.27239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eq2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0060) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.810 |
| Number of reflections | 197672 | |
| <I/σ(I)> | 15 | 1.8 |
| Completeness [%] | 97.0 | 92 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
