2X7W
Crystal structure of Thermotoga maritima endonuclease IV in the presence of cadmium and zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-07 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 61 |
| Unit cell lengths | 123.190, 123.190, 35.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.320 - 2.360 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qtw |
| RMSD bond length | 0.023 |
| RMSD bond angle | 0.794 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.100 | 0.170 |
| Number of reflections | 12749 | |
| <I/σ(I)> | 13.1 | 6.2 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1 M SODIUM CHLORIDE, 0.1 M BICINE PH 9.0, 20%(V/V) POLYETHYLENE GLYCOL MONOMETHYL ETHER 550 |
