2X7M
Crystal structure of Archaemetzincin (amzA) from Methanopyrus kandleri at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.510, 55.080, 58.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.030 - 1.500 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.620 |
| Number of reflections | 26660 | |
| <I/σ(I)> | 19.57 | 2.52 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 6.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | PH 7.6 |
