2X79

Inward facing conformation of Mhp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-13
Detector	ADSC QUANTUM 315
Spacegroup name	P 61
Unit cell lengths	173.888, 173.888, 74.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.388 - 3.800
R-factor	0.2773
Rwork	0.273
R-free	0.31260
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.115
RMSD bond angle	1.037
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.940
High resolution limit [Å]	3.800	3.800
Rmerge	0.080	0.690
Number of reflections	47973
<I/σ(I)>	17.6	1.4
Completeness [%]	97.0	96
Redundancy	3.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		24-28% PEG300, 100 MM NACL AND 100 MM BICINE PH9.0