2X78
Human foamy virus integrase - catalytic core.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.800, 98.040, 240.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.020 - 2.000 |
| R-factor | 0.21485 |
| Rwork | 0.212 |
| R-free | 0.27208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x74 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 38236 | |
| <I/σ(I)> | 16.4 | 2 |
| Completeness [%] | 91.2 | 53.9 |
| Redundancy | 6.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM SODIUM CITRATE, PH=5, 14% PEG 8000, 10% GLUCOSE, 5% MPD, 4MG/ML PROTEIN |
