2X74
Human foamy virus integrase - catalytic core.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2008-09-12 |
| Detector | ADSC CCD |
| Wavelength(s) | 1.033, 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.180, 89.110, 177.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.580 - 2.340 |
| R-factor | 0.23633 |
| Rwork | 0.233 |
| R-free | 0.29391 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.580 | 2.460 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 57496 | |
| <I/σ(I)> | 15.1 | 2 |
| Completeness [%] | 99.0 | 95.4 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8-2M AMMONIUM FORMATE, 100MM HEPES PH 7.5, 10-15% GLYCEROL |
