2X70
Crystal structure of MHC CLass I HLA-A2.1 bound to a photocleavable peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.979, 81.801, 79.706 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 19.925 - 2.000 |
| R-factor | 0.1623 |
| Rwork | 0.159 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.920 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 53033 | |
| <I/σ(I)> | 9.8 | 2.9 |
| Completeness [%] | 97.2 | 97.2 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | VAPOUR DIFFUSION HANGING DROP AT 293K: 2 MICROLITER OF 5 MG/ML PROTEIN IN 0.1 M MES PH 6.0 WAS MIXED WITH 2 MICROLITER OF 0.1 M MES PH 6.5, 20-22% PEG 1500. SEEDING WAS USED TO GET BIGGER CRYSTALS |
