2X6X

Tailspike protein mutant D339N of E.coli bacteriophage HK620 in complex with hexasaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-02
Detector	MARRESEARCH
Spacegroup name	P 3 2 1
Unit cell lengths	74.230, 74.230, 174.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.120 - 1.480
R-factor	0.15894
Rwork	0.158
R-free	0.18537
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vji
RMSD bond length	0.010
RMSD bond angle	1.375
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.120	1.520
High resolution limit [Å]	1.480	1.480
Rmerge	0.050	0.470
Number of reflections	87697
<I/σ(I)>	17.1	2.9
Completeness [%]	98.6	71.5
Redundancy	4.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	VAPOR DIFFUSION, HANGING DROP:PROTEIN CONCENTRATION 8MG/ML. BUFFER: 40MM TRIS, PH7.8,2MM EDTA,0.2M NACL.RERVOIR: 100MM TRIS PH8.5,3.5M NA-FORMIATE. DROPLET 1.5:1.5 MICRO LITER, 0.3 MICRO LITER 33MM HEXASACCHARIDE.TEMPERATURE 20 DEGREE CELSIUS.