2X6R
Crystal structure of trehalose synthase TreT from P.horikoshi produced by soaking in trehalose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 297 |
| Collection date | 2005-12-01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.852, 63.067, 92.492 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.200 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xa1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.810 | 2.390 |
| High resolution limit [Å] | 2.200 | 2.250 |
| Rmerge | 0.080 | 0.160 |
| Number of reflections | 42278 | |
| <I/σ(I)> | 11.4 | 11.4 |
| Completeness [%] | 89.9 | 85 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 3350 25%, 0.2M MGCL2, 0.1M SODIUM HEPES BUFFER |
