2X6H
THE CRYSTAL STRUCTURE OF THE DROSOPHILA CLASS III PI3-KINASE VPS34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 110.600, 154.790, 243.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.360 - 2.900 |
| R-factor | 0.242 |
| Rwork | 0.240 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8x |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.656 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.360 | 3.060 |
| High resolution limit [Å] | 2.570 | 2.900 |
| Rmerge | 0.100 | 0.880 |
| Number of reflections | 44562 | |
| <I/σ(I)> | 5.77 | 0.86 |
| Completeness [%] | 95.8 | 84.5 |
| Redundancy | 3.39 | 2.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLISED IN 0.88M AMMONIUM SULPHATE, 100MM DI-POTASSIUM HYDROGEN PHOSPHATE AND 100MM DI-SODIUM HYDROGEN PHOSPHATE (TITRATED TO PH 7.5 WITH ORTHOPHOSPHORIC ACID). |
